Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Migration01:09

Cell Migration

19.2K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
19.2K
Cell Migration01:19

Cell Migration

7.5K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
7.5K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

5.7K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
5.7K
Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

6.2K
Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
6.2K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

4.0K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
4.0K
Actin Polymerization and Cell Motility01:13

Actin Polymerization and Cell Motility

7.2K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
7.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering human peritoneum in vitro: A novel microfluidic platform for modeling peritoneal physiology and pathophysiology.

Bioengineering & translational medicine·2026
Same author

Bio-inspired micro-architected mechanochromic materials with radiative signature modulation.

Materials horizons·2026
Same author

Deep-sequencing-guided library screening and profiling of AAV capsids in the primate retina.

Molecular therapy. Advances·2026
Same author

Drug Proarrhythmic Evaluation in a High Throughput Cardiac New Approach Methodology.

bioRxiv : the preprint server for biology·2026
Same author

Optimized mechano-fluidic metamaterials inspired by deep-sea sponges.

Nature communications·2026
Same author

Lymphoid-Tissue-on-Chip Recapitulates Human Antibody Responses In Vitro.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Related Experiment Video

Updated: Apr 6, 2026

Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy
12:26

Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy

Published on: January 29, 2022

6.6K

Directing cell migration and organization via nanocrater-patterned cell-repellent interfaces.

Hojeong Jeon1,2, Sangmo Koo1, Willie Mae Reese3

  • 1Laser Thermal Laboratory, Department of Mechanical Engineering, University of California, Berkeley, California 94720, USA.

Nature Materials
|July 28, 2015
PubMed
Summary

This study demonstrates how nanoscale crater patterns on surfaces can control cell behavior, influencing cell migration and organization by altering focal adhesions. This method offers a new way to direct cell localization without chemicals.

More Related Videos

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy
13:10

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

Published on: April 4, 2013

13.1K
Control of Cell Geometry through Infrared Laser Assisted Micropatterning
11:04

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Published on: July 10, 2021

3.9K

Related Experiment Videos

Last Updated: Apr 6, 2026

Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy
12:26

Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy

Published on: January 29, 2022

6.6K
Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy
13:10

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

Published on: April 4, 2013

13.1K
Control of Cell Geometry through Infrared Laser Assisted Micropatterning
11:04

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Published on: July 10, 2021

3.9K

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Surface Engineering

Background:

  • Extensive research exists on cell adhesion to nanostructured surfaces.
  • Limited data is available on how nanostructures repel cells and direct cell migration or colony organization.

Purpose of the Study:

  • To investigate how nanostructured surfaces can repel cells and guide cell migration and organization.
  • To explore the relationship between nanostructure geometry (aspect ratio, pitch) and cell behavior.

Main Methods:

  • Utilized multiphoton ablation lithography to create nanoscale craters on surfaces.
  • Varied crater aspect ratios and pitches to systematically alter surface topography.
  • Analyzed changes in cell focal adhesion size, distribution, morphology, and migration patterns.

Main Results:

  • Nanostructured surfaces altered focal adhesion size and distribution, impacting cell morphology and migration.
  • Nanocrater pitch was found to disrupt focal adhesion formation, favoring migration towards regions with higher pitch and greater planar area.
  • Demonstrated the ability to create specific cellular patterns (circular, striped) by designing surfaces with variable pitch and constant crater dimensions.

Conclusions:

  • Nanostructured surfaces can effectively control cell behavior, including repulsion and directed migration.
  • Surface topography, specifically nanocrater pitch, is a key factor in regulating cell-surface interactions and organization.
  • This chemical-free surface patterning approach provides a versatile method for manipulating cell localization and arrangement on various materials.