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Data for proteomic analysis of murine cardiomyocytic HL-1 cells treated with siRNA against tissue factor
Maura Brioschi1, Sabrina Lento1, Simona Barcella1
1Centro Cardiologico Monzino IRCCS, Milano, Italy.
Abstract:
This data article is related to the research article entitled Proteomics of Tissue Factor silencing in cardiomyocytic cells reveals a new role for this coagulation factor in splicing machinery control by Lento et al. [1]. Tissue Factor (TF) is a key player in the coagulation cascade, but it has additional functions ranging from angiogenesis, tumour invasion and, in the heart, the maintenance of the integrity of cardiac cells. This article reports the nano-LC-MS(E) analysis of the cardiomyocytic HL-1 cell line proteome and describes the results obtained from a Gene Ontology analysis of those proteins affected by TF-gene silencing.
Insights
This study investigated Tissue Factor (TF) in heart cells, revealing its role beyond blood clotting. Silencing TF in cardiac cells uncovered its involvement in controlling the splicing machinery, impacting cell integrity.
Area of Science:
- Cardiovascular Biology
- Proteomics
- Molecular Biology
Background:
- Tissue Factor (TF) is crucial for coagulation.
- TF also plays roles in angiogenesis, tumor invasion, and cardiac cell integrity.
- Its function in cardiac splicing machinery is not well understood.
Purpose of the Study:
- To analyze the proteome of cardiomyocytic cells with silenced TF.
- To identify proteins affected by TF gene silencing.
- To explore TF's novel role in splicing machinery control.
Main Methods:
- Utilized nano-LC-MS(E) for proteomic analysis.
- Applied Gene Ontology analysis to identify affected proteins.
- Investigated TF gene silencing in the HL-1 cardiomyocytic cell line.
Main Results:
- Identified proteins significantly altered by TF gene silencing.
- Gene Ontology analysis highlighted impacts on splicing-related proteins.
- Data article provides comprehensive proteomic dataset.
Conclusions:
- TF silencing affects the cardiac cell proteome, particularly proteins involved in splicing.
- Suggests a novel role for TF in regulating splicing machinery in the heart.
- This data article supports further research into TF's non-hemostatic functions.
