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Transfer RNA Synthesis

Transfer RNA Synthesis

One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...

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Updated: Apr 6, 2026

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

A Versatile Approach Towards Nucleobase-Modified Aptamers.

Fabian Tolle¹, Gerhard M Brändle¹, Daniel Matzner¹

¹Life and Medical Sciences Institute, University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn (Germany).

Angewandte Chemie (International Ed. in English)

|July 31, 2015

Summary

This summary is machine-generated.

A new method expands the chemical diversity of nucleic acid libraries, creating modified aptamers with superior recognition abilities. This versatile approach broadens the scope of in vitro selection for novel therapeutic and diagnostic targets.

Keywords:

GFP SELEX aptamers molecular evolution nucleic acids

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Area of Science:

Biochemistry and Molecular Biology
Chemical Biology
Nucleic Acid Chemistry

Background:

Current limitations in nucleic acid library modification restrict the development of aptamers with diverse functionalities.
The need for novel aptamers with enhanced recognition properties for challenging biological targets is growing.

Purpose of the Study:

To develop a versatile and modular method for expanding the chemical space of nucleic acid libraries.
To enable the generation of nucleobase-modified aptamers with unprecedented recognition capabilities.
To overcome current limitations in aptamer development for previously unaddressable targets.

Main Methods:

Development of a novel modular strategy for chemical modification of nucleic acid libraries.
Utilizing reintroduction of modifications after enzymatic replication for broad applicability.
Demonstration of wide applicability across various chemical modifications.

Main Results:

Successful generation of nucleobase-modified aptamers with significantly enhanced recognition properties.
Demonstrated broad access to a wide range of chemical modifications.
Validated the versatility of the method beyond single-modification approaches.

Conclusions:

The developed method offers a versatile platform for modular expansion of nucleic acid chemical space.
This advancement will accelerate the application of in vitro selection for generating aptamers against challenging targets.
Enables the creation of aptamers with tailored recognition properties for diverse applications.