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Updated: Apr 6, 2026
Microcrystallography of Protein Crystals and In Cellulo Diffraction
Published on: July 21, 2017
In Vitro/In Vivo Production of tRNA for X-Ray Studies
Clément Dégut1, Alexandre Monod, Franck Brachet
1Laboratoire de Cristallographie et RMN Biologiques, CNRS, Paris Sorbonne Cité, 4 avenue de l'Observatoire, Paris, 75006, France.
Abstract:
tRNAs occupy a central role in the cellular life, and they are involved in a broad range of biological processes that relies on their interaction with proteins and RNA. Crystallization and structure resolution of tRNA or/and tRNA/partner complexes can yield in valuable information on structural organizations of key elements of cellular machinery. However, crystallization of RNA, is often challenging. Here we review two methods to produce and purify tRNA in quantity and quality to perform X-ray studies.
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