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High performance liquid chromatographic (HPLC) determination of centchroman in human serum and application to
J K Paliwal1, R C Gupta, P K Grover
1Pharmacokinetics and Metabolism Division, Central Drug Research Institute, Lucknow, India.
Pharmaceutical Research
|December 1, 1989
Abstract:
A simple and sensitive (2 ng/ml) HPLC method with fluorescence detection has been developed to measure serum concentrations of centchroman, a new nonsteroidal antifertility agent. The method was sufficiently sensitive to follow the drug over 21 days in human volunteers. Pharmacokinetic parameters of centchroman were determined after a single oral dose of 60 mg (2 x 30-mg tablets) in two healthy female volunteers. Centchroman is slowly eliminated from serum, showing a biexponential disappearance curve from serum. The terminal half-life of centchroman in the two volunteers was 168 and 175 hr, respectively.