RNA-Sequencing Analysis of Messenger RNA/MicroRNA in a Rabbit Aneurysm Model Identifies Pathways and Genes of...

Area of Science:

• Vascular Biology
• Genomics
• Molecular Medicine

Background:

• Rabbit aneurysm models are crucial for studying intracranial aneurysms and testing embolization devices.
• Understanding the molecular mechanisms of aneurysm growth and healing is essential for clinical applications.
• RNA sequencing offers a powerful tool to explore gene expression in aneurysm biology.

Purpose of the Study:

• To identify genes and microRNAs relevant to induced rabbit aneurysm biology using RNA sequencing.
• To uncover genes and pathways of potential clinical significance in aneurysm development.
• To investigate the role of noncoding RNAs, specifically microRNAs, in aneurysm pathogenesis.

Main Methods:

• Saccular aneurysms were created in rabbits using elastase induction.
• Messenger RNA and microRNA were isolated from aneurysm and control carotid artery tissues at 12 weeks.
• RNA sequencing was performed, followed by Ingenuity Pathway Analysis for data interpretation.

Main Results:

• RNA sequencing analyzed 9396 genes, with 6.9% showing significant differential expression.
• 143 genes were down-regulated and 471 up-regulated in aneurysms compared to controls.
• 5 microRNAs were up-regulated and 3 down-regulated; pathway analysis linked genes to inflammation, migration, and coagulation.

Conclusions:

• RNA sequencing revealed differential regulation of inflammation and antigen presentation pathways in rabbit aneurysms.
• ANKRD1 and TACR1 were identified as significant genes involved in regulating matrix metalloproteinases.
• The findings provide insights into the molecular underpinnings of aneurysm biology and potential therapeutic targets.