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Accelerating peroxidase mimicking nanozymes using DNA.

Biwu Liu¹, Juewen Liu

¹Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada. liujw@uwaterloo.ca.

|August 4, 2015

Summary

DNA-capped iron oxide nanoparticles show enhanced activity as peroxidase mimics. The study reveals DNA

Area of Science:

Nanotechnology
Biochemistry
Materials Science

Background:

Iron oxide nanoparticles are utilized as peroxidase mimics.
Understanding the role of surface modifications is crucial for optimizing nanoparticle activity.

Purpose of the Study:

To investigate the mechanism behind the enhanced peroxidase-like activity of DNA-capped iron oxide nanoparticles.
To determine the influence of DNA length, sequence, and other polymer coatings on nanoparticle performance.

Main Methods:

Systematic study of DNA length and sequence effects on nanoparticle activity.
Comparison with other polymer coatings and different substrates (TMB and ABTS).
Evaluation of nanoceria activity with DNA capping.

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Main Results:

DNA-capped iron oxide nanoparticles exhibited a nearly 10-fold increase in activity for TMB oxidation compared to naked nanoparticles.
Activity enhancement was more pronounced with longer DNA strands, especially poly-cytosine.
DNA provided the highest rate enhancement among tested polymer coatings.
Similar acceleration was observed with DNA-capped nanoceria.
DNA inhibited the oxidation of the negatively charged ABTS substrate.

Conclusions:

The negatively charged phosphate backbone and bases of DNA enhance TMB binding to iron oxide nanoparticles.
This enhanced binding facilitates the oxidation reaction catalyzed by the nanoparticles in the presence of hydrogen peroxide.
DNA capping is a promising strategy for improving the performance of peroxidase-mimicking nanoparticles.