Determination of ABO blood group genotypes using the real‑time loop‑mediated isothermal amplification method

Area of Science:

• Molecular Biology
• Genetics
• Clinical Diagnostics

Background:

• ABO genotyping is crucial for blood transfusions, forensics, and disease association studies.
• Current genotyping methods can be time-consuming or require specialized equipment.

Purpose of the Study:

• To develop a novel, rapid, and accurate method for ABO blood group genotyping.
• To evaluate the performance of the developed method against established techniques.

Main Methods:

• Development of a loop-mediated isothermal amplification (LAMP) assay for simultaneous detection of six ABO genotypes.
• Validation using 101 blood samples, comparing results with serological typing and direct sequencing.

Main Results:

• The LAMP assay successfully determined all six ABO genotypes (AA, AO, BB, BO, AB, OO) within 40 minutes at a constant 62°C.
• Results showed perfect concordance with serological phenotyping and direct sequencing, demonstrating high accuracy.
• The method is cost-effective and suitable for point-of-care applications.

Conclusions:

• A novel LAMP-based method provides a rapid, accurate, and cost-effective solution for ABO genotyping.
• This technique offers a valuable alternative or supplement to conventional ABO typing methods.
• The assay's speed and simplicity make it suitable for diverse clinical and forensic settings.