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Updated: Apr 6, 2026
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Published on: May 12, 2022
Purification Methodology for Viable and Infective Plasmodium vivax Gametocytes That Is Compatible with
Omaira Vera1, Paula Brelas de Brito2, Letusa Albrecht3
1Departamento de Genética, Evolução e Bioagentes, Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, Brazil Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Gerência de Malária, Manaus, Amazonas, Brazil.
Abstract:
Significant progress toward the control of malaria has been achieved, especially regarding Plasmodium falciparum infections. However, the unique biology of Plasmodium vivax hampers current control strategies. The early appearance of P. vivax gametocytes in the peripheral blood and the impossibility of culturing this parasite are major drawbacks. Using blood samples from 40 P. vivax-infected patients, we describe here a methodology to purify viable gametocytes and further infect anophelines. This method opens new avenues to validate transmission-blocking strategies.
