Deep Mutational Scanning: A Highly Parallel Method to Measure the Effects of Mutation on Protein Function
Related Experiment Video
Updated: Apr 6, 2026
A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
Published on: July 3, 2016
Lea M Starita1, Stanley Fields2
1Department of Genome Sciences, University of Washington, Seattle, Washington 98195;
Abstract:
Deep mutational scanning is a method that makes use of next-generation sequencing technology to measure in a single experiment the activity of 10(5) or more unique variants of a protein. Because of this depth of mutational coverage, this strategy provides data that can be analyzed to reveal many protein properties. Deep mutational scanning approaches are particularly amenable to being performed in Saccharomyces cerevisiae, given the extensive toolkit of reagents and technologies available for this organism.
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