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Elucidating the Ramularia eucalypti species complex.

S I R Videira1, J Z Groenewald2, A Kolecka2

  • 1CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands; ; Wageningen University and Research Centre (WUR), Laboratory of Phytopathology, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.

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Summary
This summary is machine-generated.

This study resolves the Ramularia eucalypti species complex using multi-gene phylogeny and MALDI-TOF MS. It identifies seven distinct species, including four new ones, improving fungal identification and classification.

Keywords:
Mycosphaerellaceaeplant pathogenspecies complexsystematics

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Area of Science:

  • Mycology
  • Plant Pathology
  • Molecular Systematics

Background:

  • The genus Ramularia contains economically important phytopathogenic species.
  • Ramularia eucalypti is known to cause leaf spots on Eucalyptus.
  • Morphological and ITS-based identification of Ramularia species is often unreliable, leading to a species complex.

Purpose of the Study:

  • To resolve the taxonomic ambiguity within the Ramularia eucalypti species complex.
  • To differentiate closely related Ramularia species using a polyphasic approach.
  • To describe novel species within the R. eucalypti complex.

Main Methods:

  • A polyphasic approach combining morphology, multi-gene phylogenetic analysis (ITS, ACT, TEF1-α, HIS3, GAPDH, RPB2), and MALDI-TOF MS.
  • Phylogenetic analyses included Bayesian and parsimony methods for 44 isolates.
  • Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) was used for rapid strain identification.

Main Results:

  • Phylogenetic analyses supported the separation of seven species within R. eucalypti s.lat.
  • Four novel species (R. haroldporteri, R. glennii, R. mali, R. plurivora) and one undescribed species were identified.
  • Mycosphaerella nyssicola was reclassified as Ramularia nyssicola.
  • MALDI-TOF MS differentiated strains into three clades, aiding in the identification of clinically relevant isolates.

Conclusions:

  • The study successfully resolved the R. eucalypti species complex through a comprehensive approach.
  • The findings expand the known diversity within the Ramularia genus.
  • MALDI-TOF MS shows promise for rapid identification of Ramularia species, including those with clinical relevance.