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Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition
Published on: June 16, 2019
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Single-cell resolution of morphological changes in hemogenic endothelium
Frank L Bos1, John S Hawkins1, Ann C Zovein2
1Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA 94158, USA.
Development (Cambridge, England)
|August 6, 2015
Summary
Researchers identified the morphological changes during endothelial-to-hematopoietic transition (EHT) in developing embryos. This study correlates cell shape with RUNX1 and SOX17 gene expression, revealing key events in blood cell formation.
Area of Science:
- Developmental Biology
- Cell Biology
- Hematopoiesis
Background:
- Endothelial-to-hematopoietic transition (EHT) is crucial for forming the adult hematopoietic system during embryogenesis.
- Prospective identification of EHT-fated endothelial cells and their transition mechanisms remain unclear.
Purpose of the Study:
- To precisely define the morphological events of EHT.
- To correlate cellular morphology with RUNX1 and SOX17 transcription factor expression during EHT.
Main Methods:
- Developed a novel strategy combining immunofluorescence with scanning electron microscopy for correlative single-cell analysis.
- Utilized the ratio of RUNX1 to SOX17 immunofluorescence to identify single endothelial cells undergoing EHT.
Main Results:
- Successfully correlated specific morphological changes with EHT.
- Identified distinct cellular morphologies associated with varying RUNX1/SOX17 expression levels during the transition.
- Established a new technical resource for correlative single-cell analysis in native tissue environments.
Conclusions:
- Detailed the morphological landscape of endothelial-to-hematopoietic transition.
- Provided a method to identify single cells undergoing EHT based on morphology and transcription factor levels.
- The developed technique offers broad applicability for studying single-cell dynamics in situ.
