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Updated: Apr 5, 2026

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

Open-Source Photometric System for Enzymatic Nitrate Quantification.

B T Wittbrodt¹, D A Squires², J Walbeck²

¹Department of Materials Science & Engineering, Michigan Technological University, Houghton, MI, United States of America.

|August 6, 2015

Summary

This summary is machine-generated.

A new, low-cost, open-source photometer enables field testing for nitrate pollution using a safer method. This technology significantly reduces costs and eliminates toxic chemicals, improving water quality analysis accessibility.

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Analytical Techniques for Assaying Nitric Oxide Bioactivity

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Published on: June 18, 2012

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Last Updated: Apr 5, 2026

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

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Published on: February 16, 2022

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

Area of Science:

Environmental Science
Analytical Chemistry
Water Quality Monitoring

Background:

Nitrate pollution is a significant environmental concern due to anthropogenic sources.
Current standard methods like the Cadmium Reduction Method use toxic heavy metals.
Alternative methods, such as the EPA Nitrate Reductase Nitrate-Nitrogen Analysis Method, are effective but require expensive equipment.

Purpose of the Study:

To design and validate an inexpensive, portable, open-source photometer for field nitrate analysis.
To enable the use of the safer Nitrate Reductase Nitrate-Nitrogen Analysis Method.
To reduce the cost and complexity of water quality testing for nitrate and nitrite.

Main Methods:

Development of an open-source, handheld photometer.
Technical validation of the photometer using the Nitrate Reductase Nitrate-Nitrogen Analysis Method.
Comparison of the developed device with commercial systems.

Main Results:

The developed photometer performs comparably to commercial systems.
The material cost of the device is less than 15% of commercial alternatives.
The methodology eliminates the need for toxic chemicals and simplifies field testing.

Conclusions:

An accessible, cost-effective, and safe field testing device for nitrate analysis has been developed.
This innovation democratizes access to advanced water quality monitoring techniques.
The open-source platform facilitates wider adoption and further development in combating nitrate pollution.