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Related Experiment Videos

Ascorbic acid as a factor controlling "in vivo" its biosynthetic pathway.

L De Gara, F Tommasi, R Liso

    Bollettino Della Societa Italiana Di Biologia Sperimentale
    |October 1, 1989
    PubMed
    Summary

    Potato tuber cells regulate ascorbic acid (vitamin C) biosynthesis. When vitamin C levels are high, synthesis is blocked, but adding the final precursor rapidly increases production, indicating regulation at an early step.

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    Purification and properties of ascorbate free-radical reductase from potato tubers.

    Planta·2013

    Area of Science:

    • Biochemistry
    • Plant Physiology

    Background:

    • Ascorbic acid (vitamin C) is a vital antioxidant in plants.
    • Potato tuber tissue exhibits varying capacities for ascorbic acid biosynthesis.

    Purpose of the Study:

    • To investigate the regulatory mechanisms of ascorbic acid biosynthesis in potato tuber cells.
    • To determine the correlation between endogenous ascorbic acid levels and biosynthetic capacity.

    Main Methods:

    • Measuring ascorbic acid content in potato tuber cells.
    • Assessing ascorbic acid biosynthesis capacity by adding precursors like glucose and galactono-gamma-lactone.
    • Evaluating the activity of the final step in the pathway (galactone oxidase).

    Main Results:

    • Ascorbic acid biosynthesis capacity is inversely correlated with endogenous ascorbic acid levels.

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  • At high endogenous ascorbic acid concentrations, biosynthesis is minimal.
  • Adding glucose had no effect, while galactono-gamma-lactone addition stimulated significant ascorbic acid synthesis.
  • The final enzymatic step (galactone oxidase) was consistently active and not rate-limiting.

    • Conclusions:

    • Ascorbic acid biosynthesis in potato tubers is regulated at an early, rate-limiting step.
    • The pathway is not regulated at the final galactone oxidase step.
    • Endogenous ascorbic acid levels act as a feedback signal to control initial biosynthesis stages.