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The ITS2 Database
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ITS2 Database V: Twice as Much.

Markus J Ankenbrand1, Alexander Keller1, Matthias Wolf2

  • 1Department of Animal Ecology and Tropical Biology, Julius Maximilian University, Würzburg, Germany.

Molecular Biology and Evolution
|August 8, 2015
PubMed
Summary
This summary is machine-generated.

The updated internal transcribed spacer 2 (ITS2) database now includes nearly double the sequences, enhancing phylogenetic analyses and DNA barcoding across major eukaryotic clades. This expansion provides broader coverage for evolutionary studies.

Keywords:
barcodingdatabaseinternal transcribed spacerphylogenysequence-structuretoolbox

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Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Evolutionary Biology

Background:

  • The internal transcribed spacer 2 (ITS2) is a crucial marker for eukaryotic phylogenetic analysis.
  • The ITS2 database has been a vital resource for reference sequences and secondary structures but was last updated in 2011.
  • An updated database is needed to reflect current knowledge and expand its utility.

Purpose of the Study:

  • To present a major update to the ITS2 database, significantly increasing its data content and taxonomic representation.
  • To improve the utility of ITS2 as a phylogenetic marker and DNA barcode through enhanced data coverage.
  • To provide a more comprehensive resource for researchers studying eukaryotic evolution.

Main Methods:

  • Compilation and integration of new sequence data into the existing ITS2 database framework.
  • Expansion of sequence entries, almost doubling the number of available entities.
  • Inclusion of data for underrepresented and new eukaryotic groups.

Main Results:

  • The updated database nearly doubles the number of ITS2 sequence entries.
  • Increased representation of taxa across all major eukaryotic clades.
  • Enhanced coverage for previously underrepresented and newly added groups.

Conclusions:

  • The updated ITS2 database provides significantly broader taxonomic coverage, improving its reliability for phylogenetic analyses.
  • The expanded dataset strengthens the utility of ITS2 as a DNA barcode for species identification and evolutionary studies.
  • This resource advancement supports more robust and comprehensive research in eukaryotic molecular evolution.