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Ischemic postconditioning altered microRNAs in human valve replacement
Yang Gao1, Rimao Huang1, Ri Chen1
1Department of Cardiothoracic Surgery, Xiangya Hospital, Central South University, Changsha, P.R. China.
Background:
Although the involvement of microRNAs (miRNAs) has been intensively studied in myocardial infarction, there is no report on the regulation of miRNAs by ischemic postconditioning in patients undergoing cardiac surgery. We aim to explore the regulation of miRNAs by ischemic postconditioning in double valve replacement.
Materials And Methods:
In this prospective, controlled clinical study, consecutive 30 patients undergoing double valve replacement were enrolled. The patients were randomized into two groups, namely an ischemic postconditioning (IPO) group (n = 15) and a control (CON) group (n = 15). For ethical considerations, samples of right atrial muscle were harvested, respectively, 10 min before cardiopulmonary bypass (pre-CPB) and 5 min after aortic declamping (post-CPB) for analysis of miRNAs, genes and apoptosis.
Results:
Compared with the CON group, miR-1 was downregulated, whereas miR-21 was upregulated, and BCL2 messenger RNA (mRNA) was upregulated, whereas BAX mRNA and programmed cell death 4 mRNA remained unchanged in the IPO group. Likewise, a significant increase in BCL2 protein and a striking decrease in BAX protein were observed in the IPO group when compared with those in the CON group. The IPO group showed a significantly smaller increase of terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling-positive myocytes after CPB than CON group.
Conclusions:
Ischemic postconditioning could regulate miR-1, miR-21, and downstream effectors and resulted in actual attenuation of apoptosis in patients undergoing valvular heart surgery.
Insights
Ischemic postconditioning regulates microRNAs (miRNAs) and their targets, reducing apoptosis in patients undergoing valve surgery. This study explores miRNA regulation by postconditioning in double valve replacement patients.
Area of Science:
- Cardiovascular Research
- Molecular Biology
- Surgical Innovation
Background:
- MicroRNAs (miRNAs) role in myocardial infarction is known, but their regulation by ischemic postconditioning in cardiac surgery patients is unstudied.
- This study investigates miRNA regulation by ischemic postconditioning during double valve replacement surgery.
Purpose of the Study:
- To explore the regulation of specific microRNAs (miRNAs) by ischemic postconditioning (IPO) in patients undergoing double valve replacement.
- To assess the impact of IPO on downstream gene and protein expression related to apoptosis.
Main Methods:
- Prospective, controlled clinical study involving 30 patients undergoing double valve replacement.
- Patients randomized into an ischemic postconditioning (IPO) group (n=15) and a control (CON) group (n=15).
- Right atrial muscle samples collected pre- and post-cardiopulmonary bypass (CPB) for miRNA, gene, and apoptosis analysis.
Main Results:
- IPO group showed altered miR-1 (downregulated) and miR-21 (upregulated) compared to CON.
- BCL2 mRNA and protein increased, while BAX mRNA and protein decreased in the IPO group.
- The IPO group exhibited a significantly smaller increase in apoptotic myocytes post-CPB.
Conclusions:
- Ischemic postconditioning effectively regulates miR-1 and miR-21 in patients undergoing valvular heart surgery.
- IPO influences downstream apoptotic pathways, leading to reduced myocyte apoptosis.
- These findings suggest IPO as a potential protective strategy in cardiac surgery.
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