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Conservative Site-specific Recombination and Phase Variation

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Updated: Apr 5, 2026

The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker

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Published on: January 11, 2011

Cell type-specific manipulation with GFP-dependent Cre recombinase.

Jonathan C Y Tang^1,2,3, Stephanie Rudolph⁴, Onkar S Dhande^5,6,7

¹Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts, USA.

Nature Neuroscience

|August 11, 2015

Summary

This summary is machine-generated.

Scientists developed CRE-DOG, a novel method using Green Fluorescent Protein (GFP) to enable genetic manipulation in specific cells. This tool allows for targeted gene expression and optogenetic control in GFP-labeled cells.

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Last Updated: Apr 5, 2026

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Area of Science:

Molecular Biology
Neuroscience
Genetics

Background:

Transgenic GFP reporter lines visualize specific cell populations.
Functional studies require methods to genetically manipulate these visualized cells.

Purpose of the Study:

To develop a method for genetic manipulation in GFP-labeled cells.
To enable targeted gene expression and optogenetic control using GFP.

Main Methods:

Developed Cre recombinase dependent on GFP (CRE-DOG), a split-component system.
Utilized plasmid electroporation and AAV viral vectors for delivery.
Applied CRE-DOG to multiple GFP mouse lines.

Main Results:

Achieved effective and selective recombination in GFP-labeled cells.
Demonstrated optogenetic control of targeted neurons.
Showcased GFP's potential to reconstitute protein activity.

Conclusions:

CRE-DOG provides a versatile tool for selective gene manipulation in GFP(+) cells.
The strategy may be broadly applicable to various proteins beyond GFP.
This advances functional studies in transgenic reporter lines.