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Updated: Apr 5, 2026

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
Published on: March 16, 2022
Premature termination of GAT1 transcription explains paradoxical negative correlation between nitrogen-responsive
Isabelle Georis, Georis Isabelle1, Jennifer J Tate
1a Yeast Physiology ; Institut de Recherches Microbiologiques J. M. Wiame ; Laboratoire de Microbiologie Université Libre de Bruxelles ; Brussels , Belgium.
Abstract:
The first step in executing the genetic program of a cell is production of mRNA. In yeast, almost every gene is transcribed as multiple distinct isoforms, differing at their 5' and/or 3' termini. However, the implications and functional significance of the transcriptome-wide diversity of mRNA termini remains largely unexplored. In this paper, we show that the GAT1 gene, encoding a transcriptional activator of nitrogen-responsive catabolic genes, produces a variety of mRNAs differing in their 5' and 3' termini. Alternative transcription initiation leads to the constitutive, low level production of 2 full length proteins differing in their N-termini, whereas premature transcriptional termination generates a short, highly nitrogen catabolite repression- (NCR-) sensitive transcript that, as far as we can determine, is not translated under the growth conditions we used, but rather likely protects the cell from excess Gat1.
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