Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Raman Spectroscopy: Overview

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dietary omega-6 lipids promote post-injury aberrant bone formation in obesity.

The Journal of clinical investigation·2026

Same author

Sensory nerves protect against preclinical tendinopathic changes through FGF1 signaling.

Science translational medicine·2026

Same author

VEGF-D-induced intraosseous lymphangiogenesis drives site-specific heterotopic bone resorption.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Airborne <sup>1</sup>O<sub>2</sub> Delivery via a Superhydrophobic Dressing as a Pathway to Next-Generation Wound Therapies, an In Vivo Murine Burn Model Study.

Advanced healthcare materials·2026

Same author

Using the consolidated framework for implementation research to evaluate a model of community-engaged research in advance care planning.

PloS one·2026

Same author

Wound Management and Surgery for Calcific Uremic Arteriolopathy (Calciphylaxis): A Retrospective Observational Cohort Study.

International wound journal·2026

Search research articles

Related Experiment Video

Updated: Apr 5, 2026

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

Raman spectroscopy for label-free identification of calciphylaxis.

William R Lloyd¹, Shailesh Agarwal², Sagar U Nigwekar³

¹University of Michigan, Department of Chemistry, 930 North University Avenue, Ann Arbor, Michigan 48109, United States.

Journal of Biomedical Optics

|August 12, 2015

Summary

Raman spectroscopy offers a noninvasive method to detect calciphylaxis mineral deposits. This rapid, point-of-care technology can identify calcified vasculature and soft tissues, aiding in diagnosis and disease management.

More Related Videos

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Published on: September 26, 2019

Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging

Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging

Published on: November 24, 2021

Related Experiment Videos

Last Updated: Apr 5, 2026

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Published on: September 26, 2019

Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging

Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging

Published on: November 24, 2021

Area of Science:

Biomedical Engineering
Medical Diagnostics
Materials Science

Background:

Calciphylaxis is a severe condition causing painful, calcified lesions in vasculature and soft tissues.
Current diagnostic methods, like invasive biopsies, are destructive, time-consuming, and risk infection.
Identifying small calcifications within large wound beds poses a significant diagnostic challenge.

Purpose of the Study:

To evaluate Raman spectroscopy as a noninvasive, point-of-care diagnostic tool for calciphylaxis.
To assess the capability of Raman spectroscopy in detecting ectopic calcified mineral and defining disease margins.
To establish a rapid, label-free method for identifying calciphylaxis mineral.

Main Methods:

Debrided calciphylactic tissue samples from six patients were collected.
Microcomputed tomography (micro-CT) was used to confirm the presence of extensive calcified deposits.
Raman spectroscopy was applied to analyze the confirmed calcified deposits.

Main Results:

Micro-CT confirmed significant calcified deposits in three of the six tissue specimens.
Raman spectroscopy analysis of these deposits revealed spectral signatures consistent with carbonated apatite.
The findings align with established literature on calciphylaxis mineral composition.

Conclusions:

Raman spectroscopy demonstrates significant potential as a noninvasive diagnostic technique for calciphylaxis.
This technology can rapidly identify calciphylaxis mineral in a clinical setting.
Further development could lead to improved diagnostic accuracy and patient outcomes for calciphylaxis.