Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

3.4K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
3.4K
Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

19.5K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
19.5K
COP Coated Vesicles00:59

COP Coated Vesicles

18.6K
Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
18.6K
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

4.4K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
4.4K
The Movement of Organelles and Vesicles01:43

The Movement of Organelles and Vesicles

7.3K
In eukaryotic cells,  cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...
7.3K
SNAREs and Membrane Fusion01:43

SNAREs and Membrane Fusion

13.6K
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
13.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reprogrammable Bistable Metasurface for Arbitrary Electromagnetic Wave Manipulation.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Regenerable PVA Hydrogel-Functionalized Optical Fiber Sensor for Ultra-Trace Detection of Berberine Hydrochloride.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Spatiotemporal Mapping of Field-Driven Electron Spillover across ZnO Facets.

Angewandte Chemie (International ed. in English)·2026
Same author

Automatic classification of circulating blood cell clusters based on multi-channel flow cytometry imaging.

Engineering applications of artificial intelligence·2026
Same author

First-trimester plasma targeted metabolomics for eicosanoids reveals predictive potential and preventive targets for severe preeclampsia: a nested case-control study.

BMC pregnancy and childbirth·2026
Same author

Achieving Highly Sensitive In Situ Detection of Trace Starch with a Hydrogel-Functionalized MZI Optical Fiber Biosensor.

Langmuir : the ACS journal of surfaces and colloids·2026

Related Experiment Video

Updated: Apr 5, 2026

Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum
07:49

Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum

Published on: January 22, 2019

8.4K

Nanotube-Enabled Vesicle-Vesicle Communication: A Computational Model.

Liuyang Zhang1, Xianqiao Wang1

  • 1College of Engineering and NanoSEC, University of Georgia, Athens, Georgia 30602, United States.

The Journal of Physical Chemistry Letters
|August 13, 2015
PubMed
Summary

Researchers designed artificial cell communication channels using rotational nanotubes (RNTs) and vesicles. These RNTs facilitate substance transfer between cells, offering a novel synthetic biology approach.

Keywords:
biological cellular networkchanneldissipative particle dynamicsrotational nanotubes

More Related Videos

Synthesis of Compound Giant Unilamellar Vesicles: A Biomimetic Model of Nucleate Cells
10:10

Synthesis of Compound Giant Unilamellar Vesicles: A Biomimetic Model of Nucleate Cells

Published on: July 3, 2025

1.2K
Pulling Membrane Nanotubes from Giant Unilamellar Vesicles
06:26

Pulling Membrane Nanotubes from Giant Unilamellar Vesicles

Published on: December 7, 2017

11.7K

Related Experiment Videos

Last Updated: Apr 5, 2026

Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum
07:49

Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum

Published on: January 22, 2019

8.4K
Synthesis of Compound Giant Unilamellar Vesicles: A Biomimetic Model of Nucleate Cells
10:10

Synthesis of Compound Giant Unilamellar Vesicles: A Biomimetic Model of Nucleate Cells

Published on: July 3, 2025

1.2K
Pulling Membrane Nanotubes from Giant Unilamellar Vesicles
06:26

Pulling Membrane Nanotubes from Giant Unilamellar Vesicles

Published on: December 7, 2017

11.7K

Area of Science:

  • Biophysics
  • Synthetic Biology
  • Materials Science

Background:

  • Cell-to-cell communication is crucial for multicellular organisms, relying on intrinsic pathways like tunneling nanotubes and gap junctions.
  • Designing artificial communication channels between cells presents a significant challenge in synthetic biology.

Discussion:

  • Dissipative particle dynamics (DPD) simulations were used to study the interaction between rotational nanotubes (RNTs) and vesicles.
  • RNTs were shown to induce local disturbances, promoting vesicle translocation and the formation of RNT-vesicle networks.
  • Ligand patterning on RNTs allows for targeted design of communication pathways.

Key Insights:

  • Rotational nanotubes (RNTs) can act as artificial channels for intercellular communication.
  • Patterned ligand coatings on RNTs enable the creation of specific RNT-vesicle networks.
  • RNTs facilitate substance transfer between vesicles, mimicking natural cell communication.

Outlook:

  • This work provides a molecular design framework for creating synthetic cell-to-cell communication channels.
  • Findings can guide the development of novel biomaterials for cellular engineering and drug delivery.
  • Further research can explore the scalability and efficiency of RNT-based communication systems.