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Proteomic analysis on zoxamide-induced sensitivity changes in Phytophthora cactorum
Xinyue Mei1, Min Yang1, Bingbing Jiang1
1Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, Yunnan 650201, China.
Pesticide Biochemistry and Physiology
|August 13, 2015
Summary
Phytophthora cactorum shows low resistance risk to the fungicide zoxamide. However, continuous application may decrease zoxamide
Area of Science:
- Agricultural Science
- Plant Pathology
- Mycology
Background:
- Zoxamide is a key fungicide for managing oomycete diseases.
- Understanding pathogen sensitivity and resistance development is crucial for effective disease management.
Purpose of the Study:
- To determine the baseline sensitivity of Phytophthora cactorum to zoxamide.
- To assess the risk of zoxamide resistance development in P. cactorum.
Main Methods:
- Baseline sensitivity was established using mycelial growth inhibition assays.
- Resistance risk was assessed via ultraviolet irradiation and fungicide taming.
- Proteomic and semi-quantitative PCR analyses were used to investigate molecular mechanisms.
Main Results:
- All 73 P. cactorum isolates were sensitive to zoxamide (EC50: 0.04–0.29 mg/L).
- Stable zoxamide-resistant mutants were not obtained; however, sensitivity decreased with successive treatments.
- Cross-resistance to flumorph and cymoxanil was observed, linked to proteins involved in detoxification and resistance pathways.
Conclusions:
- Phytophthora cactorum exhibits a low intrinsic risk of developing resistance to zoxamide.
- Induced resistance can reduce zoxamide efficacy with continuous application, potentially impacting disease management strategies.
