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Aspirin response: Differences in serum thromboxane B2 levels between clinical studies
Charlotte Brun1,2, Youssef Daali2,3, Christophe Combescure4
1a Division of General Internal Medicine , Geneva University Hospitals , Geneva , Switzerland .
Platelets
|August 14, 2015
Summary
Serum thromboxane B2 (TxB2) levels vary significantly in aspirin-treated patients. Factors like BMI, age, and aspirin dose influence TxB2, but unexplained cohort differences persist, questioning its prognostic value.
Area of Science:
- Cardiovascular Medicine
- Pharmacology
- Biomarker Research
Background:
- Serum thromboxane B2 (TxB2) is a key marker for aspirin's platelet inhibition.
- Significant inter-cohort variability in TxB2 levels exists among aspirin-treated patients.
Purpose of the Study:
- To identify factors contributing to serum TxB2 level differences between the ADRIE and BOSTON study cohorts.
- To investigate the influence of patient characteristics and assay methods on TxB2 variability.
Main Methods:
- Comparative analysis of TxB2 levels using ELISA and liquid chromatography-tandem mass spectrometry (MS) in subgroups.
- Multivariate analysis of a combined dataset (n=1342) from ADRIE and BOSTON cohorts.
- Assessment of factors including BMI, age, gender, aspirin dose, NSAID use, and C-reactive protein.
Main Results:
- No systematic bias was found between ELISA and MS TxB2 measurements.
- Median TxB2 levels differed substantially between ADRIE (7 ng/ml) and BOSTON (0.6 ng/ml) cohorts.
- BMI, age, gender, aspirin dose, time since intake, NSAID use, platelet count, and CRP were associated with TxB2 levels.
- Cohort differences in TxB2 remained significant after adjusting for patient characteristics.
Conclusions:
- Patient characteristics explain some TxB2 variability but do not account for inter-cohort differences.
- Unexplained variations in serum TxB2 highlight the need for further research.
- The role of serum TxB2 as a prognostic factor or marker of therapeutic adherence requires additional investigation.
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