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Photoluminescence: Applications01:14

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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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Atomic spectroscopy is a vital tool in elemental analysis, both qualitatively and quantitatively. It can be broadly divided into optical spectroscopy, mass spectroscopy, and X-ray spectroscopy methods. The optical spectroscopic methods are atomic absorption spectroscopy (AAS), atomic emission spectroscopy (AES), and atomic fluorescence spectroscopy (AFS). The first step in all three methods is atomization, where the solid, liquid, or solution-phase samples are converted into gas-phase atoms and...
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The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
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Atomic Fluorescence Spectroscopy01:29

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Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Detection and Removal of Tooth-Colored Composite Resin Using the Fluorescence-Aided Identification Technique
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Application of Fluorescence Spectroscopy to Forensic Science.

J A Siegel1

  • 1School of Criminal Justice, Michigan State University, East Lansing, MI, USA.

Forensic Science Review
|August 14, 2015
PubMed
Summary
This summary is machine-generated.

Ultraviolet and infrared fluorescence techniques, enhanced by lasers, are revolutionizing forensic science. These methods offer advanced visualization for fingerprints, ink analysis, and other evidence types, improving accuracy and detail.

Keywords:
Drugsfibersfingerprintsfluorescenceglassgunshot residuesluminescencepetroleum productsquestioned documents

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Area of Science:

  • Forensic Science
  • Analytical Chemistry

Background:

  • Laser-based visualization has significantly advanced forensic science applications.
  • Fluorescence techniques, both ultraviolet (UV) and infrared (IR), have seen increased use in forensic analysis over the past decade.

Purpose of the Study:

  • To discuss the application of UV and IR fluorescence techniques in forensic science.
  • To highlight the advantages of fluorescence-based methods compared to other analytical techniques.

Main Methods:

  • Utilizing laser methods for visualization.
  • Developing novel fluorescent dyes for fingerprint development.
  • Applying fluorimetric analysis to various forensic evidence types.

Main Results:

  • Significant advancements in fingerprint visualization using laser-induced fluorescence.
  • Successful application of fluorescence in the characterization of inks for questioned-document analysis.
  • Broad utility of fluorimetric analysis across diverse forensic samples including drugs, glass, petroleum products, and biological materials.

Conclusions:

  • Fluorescence techniques, particularly when coupled with laser visualization, offer powerful analytical tools in forensic science.
  • These methods provide distinct advantages over traditional techniques for analyzing various types of forensic evidence.
  • Continued research and development in fluorescent dyes and laser technology promise further improvements in forensic investigations.