Related Experiment Video
Updated: Apr 5, 2026
A Syngeneic Murine Model of Endometriosis using Naturally Cycling Mice
Published on: November 24, 2020
Effect of PDGF-Rb antagonist imatinib on endometrial injury repairing in mouse model
Xin-Rong Wang1, Cheng-De Wang2, Xue-Mei Liu1
1Department of Reproductive Medicine, Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai 264000, China.
Objective:
To study the effects of PDGF-Rb antagonists imatinib on endometrial injury repairing in the mouse model.
Methods:
The cultured MSCs cells from male mice were marked with BrdU in vitro, and then transplanted to the female mice which suffered from radiation injury through tail vein, PDGF-Rb antagonists imatinib was injected through abdominal cavity. Four groups were arranged, which were radiation transplantation group, normal control group, imatinib intervention group and radiation control group. BrdU incorporation, SRY expression and MVD status were detected in uterus of mice.
Results:
SRY gene was negative expressed in normal control group and radiation control group. SRY gene presented positive in radiation transplantation group and imatinib intervention group; BrdU incorporation showed negative in radiation control group and normal control group which died in the early stage in mice; the incorporation of BrdU was higher in radiation transplantation group compared with imatinib intervention group; CD34 was positive on the uterus of all the four groups, which showed highest in radiation control group and lowest in radiation control group; The MVD in imatinib intervention group was lower than radiation control group; the difference of MVD was significantly compared with normal control group (P < 0.05).
Conclusions:
PDGF-Rb antagonists imatinib could inhibit the repairing function of MSCs in the endometrial lesions in mice.
Insights
PDGF-Rb antagonists imatinib inhibit mesenchymal stem cell (MSC) repair of endometrial injury in mice. This study highlights imatinib
Area of Science:
- Reproductive biology
- Stem cell research
- Pharmacology
Background:
- Endometrial injury can impair fertility.
- Mesenchymal stem cells (MSCs) show potential for tissue repair.
- Platelet-derived growth factor receptor beta (PDGF-Rb) antagonists may influence repair processes.
Purpose of the Study:
- To investigate the effect of imatinib, a PDGF-Rb antagonist, on endometrial injury repair in a mouse model.
- To assess the impact of imatinib on MSC function in endometrial repair.
Main Methods:
- Cultured male mouse MSCs were labeled with BrdU and transplanted into female mice with radiation-induced endometrial injury.
- Imatinib was administered intraperitoneally.
- Groups included radiation + transplantation, normal control, imatinib intervention, and radiation control.
- SRY expression, BrdU incorporation, and microvessel density (MVD) were analyzed in uterine tissues.
Main Results:
- SRY gene expression was positive in radiation transplantation and imatinib groups, negative in controls.
- BrdU incorporation was higher in the radiation transplantation group than the imatinib group.
- Microvessel density (MVD) was lower in the imatinib intervention group compared to the radiation control group (P < 0.05).
Conclusions:
- PDGF-Rb antagonist imatinib inhibits the repair function of MSCs in radiation-induced endometrial lesions in mice.
- Imatinib may negatively impact endometrial regeneration by affecting MSCs and vascularization.
