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Efficient fluorescence energy transfer system between fluorescein isothiocyanate and CdTe quantum dots for the
Yueshu Feng1, Liwei Liu1, Siyi Hu1
1School of Science, Changchun University of Science and Technology, Changchun, People's Republic of China.
Abstract:
We report a fluorescence resonance energy transfer (FRET) system in which the fluorescent donor is fluorescein isothiocyanate (FITC) dye and the fluorescent acceptor is CdTe quantum dot (QDs). Based on FRET quenching theory, we designed a method to detect the concentration of silver ions (Ag(+)). The results revealed a good linear trend over Ag(+) concentrations in the range 0.01-8.96 nmol/L, a range that was larger than with other methods; the quenching coefficient is 0.442. The FRET mechanism and physical mechanisms responsible for dynamic quenching are also discussed.
