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[Protective effect of ginsenoside Rg1 again PC-12 cells in OGD injury through mTOR/Akt/FoxO3 signaling pathway]
Objective:
To investigate the protective effect of ginsenoside Rg1 on oxygen-glucose deprivation (OGD) in PC-12 cells, and preliminarily discuss the potential molecular mechanism of mTOR/Akt/FoxO3 signaling pathway.
Method:
The OGD PC-12 cell model was established. The cell viability was measured by MTT assay. After the pretreatment with Rg1 with the concentration of 10, 20, 40 micromol x L(-1) for 24 h, the cell viability was observed. Lactate dehydrogenase (LDH) release, superoxide dismutase (SOD) ac- tivity and malondialdehyde (MDA) level were detected by colorimetry assay. mTOR, p-Akt(ser473), p-Akt(tjr308), Akt, p-FoxO3, FoxO3 in cytoplasm and nucleus, and total FoxO3 protein expression were detected by Western blot assay.
Result:
OGD could significantly in- hibit cell proliferation in 4-24 h in a time-dependent manner. After pretreatment for 24 h, Rg1 (20, 40 micromol x L(-1)) could notably elevate the cell viability and SOD viability and reduce the LDH release and MDA content. Besides, Rg1 also inhibited OGD-induced mTOR and p-Akt(ser473) decreases. After treatment for 6 h, OGD could reduce FoxO3 phosphorylation and promote FoxO3 in cytoplasm. This data suggested that Rg1 could protect PC-12 cell injury through mTOR/p-Akt/FoxO3 signaling pathway.
Conclusion:
Ginsenoside Rg1 could attenuate OGD-induced PC-12 cell injury. Its action mechanism may be closely related to activation of mTOR/p-Akt/FoxO3 signaling pathway.
Insights
Ginsenoside Rg1 protects PC-12 cells from oxygen-glucose deprivation (OGD) injury. It works by activating the mTOR/Akt/FoxO3 signaling pathway, reducing cell damage and improving viability.
Area of Science:
- Neuroscience
- Pharmacology
- Cell Biology
Background:
- Oxygen-glucose deprivation (OGD) models neuronal injury.
- PC-12 cells are a common model for studying neuronal damage.
- Ginsenoside Rg1 is a compound with potential therapeutic properties.
Purpose of the Study:
- To investigate the protective effects of ginsenoside Rg1 against OGD-induced injury in PC-12 cells.
- To explore the role of the mTOR/Akt/FoxO3 signaling pathway in ginsenoside Rg1's protective mechanism.
Main Methods:
- Established an OGD model in PC-12 cells.
- Assessed cell viability using MTT assay.
- Measured lactate dehydrogenase (LDH) release, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) levels.
- Analyzed protein expression of mTOR, Akt, and FoxO3 using Western blot.
Main Results:
- OGD significantly inhibited PC-12 cell proliferation.
- Ginsenoside Rg1 (20, 40 μmol/L) increased cell viability and SOD activity, while reducing LDH release and MDA content.
- Rg1 counteracted OGD-induced decreases in mTOR and p-Akt(ser473).
- OGD reduced FoxO3 phosphorylation, and Rg1 treatment modulated this effect.
Conclusions:
- Ginsenoside Rg1 attenuates OGD-induced PC-12 cell injury.
- The protective mechanism involves the activation of the mTOR/Akt/FoxO3 signaling pathway.
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