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Published on: August 14, 2014
Endurance Training Attenuates Catabolic Signals Induced by TNF-α in Muscle of Mice
Julie Rodriguez1, Rodrigo Fernández-Verdejo, Nicolas Pierre
11Institute of Neuroscience, Université Catholique de Louvain, Louvain-la-Neuve, BELGIUM; and 2ELEONOR sprl, La Hulpe, BELGIUM.
Purpose:
To determine whether endurance training attenuates the negative effects induced by an acute injection of tumor necrosis factor-α (TNF-α) in skeletal muscle of mice.
Methods:
Trained (6 wk of endurance training at 60% of maximal velocity) and untrained mice were injected with TNF-α or vehicle and killed 6 h after. Tibialis anterior muscles were analyzed using Western blot and qRT-PCR for markers of inflammation and protein synthesis/degradation.
Results:
Independently of training, TNF-α increased the mRNA of cytokines and downregulated signals linked to protein synthesis. The phosphorylation of IKKα/β and IκBα induced by TNF-α was blunted in trained mice, suggesting altered NF-κB activation. This was associated with lower induction of several markers of protein degradation (FoxO1, MURF1, MAFbx, myostatin, Gabarapl1, and LC3BII/LC3BI ratio).
Conclusions:
Endurance training protects skeletal muscle against the activation of protein degradation signaling pathways induced by TNF-α.
