Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From Fundamental Photophysics to Photocatalysis: Energy Gap Law Analysis of Anion Radical Excited States.

ACS central science·2026
Same author

Influence of blend composition on morphology and exciton-charge dynamics in MEH-PPV: PMMA thin films.

Physical chemistry chemical physics : PCCP·2026
Same author

Why Fundamental Research Matters: Lessons from Nuclear Magnetic Resonance.

ACS central science·2026
Same author

In-Cell Photoactivated Porphyrin Demetalation for Reductive Photodynamic Therapy under Hypoxia.

Journal of medicinal chemistry·2026
Same author

Introducing "In Focus: Basic Research to Broader Impacts".

ACS central science·2026
Same author

Activation of Lysozyme with Robust Flavoenzyme Activity by Amyloid Fibrillation.

ACS applied bio materials·2026

Related Experiment Video

Updated: Apr 5, 2026

In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia
06:43

In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia

Published on: September 12, 2025

1.4K

Helical Conjugated Polyelectrolyte Aggregation Induced by Biotin-Avidin Interaction.

Danlu Wu1, Fude Feng1, Dongping Xie1

  • 1Department of Chemistry, University of Florida, Gainesville, Florida 32611-7200, United States.

The Journal of Physical Chemistry Letters
|August 20, 2015
PubMed
Summary

Fluorescence correlation spectroscopy (FCS) reveals avidin-induced cross-linking in a polymer-biotin system. This forms supramolecular aggregates, enabling a sensitive sensor for biotin-avidin interactions.

Keywords:
aggregationavidin−biotinconjugated polyelectrolytefluorescence correlation spectroscopy

More Related Videos

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
12:28

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

Published on: October 15, 2016

12.2K
Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry
08:07

Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry

Published on: January 17, 2025

2.4K

Related Experiment Videos

Last Updated: Apr 5, 2026

In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia
06:43

In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia

Published on: September 12, 2025

1.4K
Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
12:28

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

Published on: October 15, 2016

12.2K
Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry
08:07

Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry

Published on: January 17, 2025

2.4K

Area of Science:

  • Polymer science
  • Bioconjugation chemistry
  • Spectroscopy

Background:

  • A helical anionic conjugated polyelectrolyte (P1) interacts with a biotin-tetramethylrhodamine (TMR) conjugate (2).
  • Previous studies confirmed TMR intercalation into the P1 helix.

Purpose of the Study:

  • To demonstrate avidin-induced cross-linking in the P1/2 system using fluorescence correlation spectroscopy (FCS).
  • To develop a novel sensor for biotin-avidin interactions based on FCS.

Main Methods:

  • Fluorescence correlation spectroscopy (FCS) to analyze diffusion times.
  • Fluorescence spectroscopy to confirm initial binding.
  • Atomic force microscopy (AFM) for aggregate visualization.

Main Results:

  • Avidin addition to the P1/2 complex caused a significant increase in diffusion time, indicating aggregate formation.
  • FCS detected supramolecular polymer aggregates due to biotin-avidin cross-linking.
  • AFM imaging corroborated the existence of these aggregates.
  • A novel sensor for biotin-avidin interaction with a detection limit below 100 pM was developed.

Conclusions:

  • FCS is a powerful tool for detecting supramolecular polymer aggregation.
  • The biotin-avidin interaction can be effectively monitored via changes in polymer complex diffusion.
  • This study presents a highly sensitive method for avidin detection.