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Related Experiment Videos

[Type II SsrI restriction endonuclease from Staphylococcus saprophyticus].

V S Dedkov, G G Prikhod'ko, L I Puchkova

    Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia
    |November 1, 1989
    PubMed
    Summary

    The restriction enzyme SsrI, isolated from Staphylococcus saprophyticus, recognizes the DNA sequence 5'-GTT decreases AAC-3'. This enzyme can be utilized in molecular biology for DNA analysis, offering an alternative to HpaI.

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    [Replication protein A as a major eukaryotic single-stranded DNA-binding protein and its role in DNA repair].

    Molekuliarnaia biologiia·2016

    Area of Science:

    • Molecular Biology
    • Enzymology
    • Genetics

    Context:

    • Restriction enzymes are crucial tools in molecular biology for DNA manipulation.
    • Understanding enzyme specificity is vital for accurate genetic research and diagnostics.
    • Staphylococcus saprophyticus is a known bacterium, but its enzymatic repertoire for DNA research is less explored.

    Purpose:

    • To determine the specific DNA recognition sequence and cleavage site of the SsrI restriction endonuclease.
    • To characterize the SsrI enzyme isolated from Staphylococcus saprophyticus.
    • To evaluate SsrI as a potential alternative to existing restriction enzymes like HpaI.

    Summary:

    • The recognition sequence for SsrI was identified as 5 eal'-GTT decreases AAC-3'.
    • The cleavage site was precisely mapped within this recognition sequence.

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  • SsrI was successfully isolated and purified from Staphylococcus saprophyticus.

    • Impact:

    • Provides a new restriction enzyme (SsrI) for molecular biology applications.
    • Offers an alternative to HpaI, potentially with different cutting efficiencies or specificities.
    • Facilitates advanced DNA analysis, cloning, and genetic engineering techniques.