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Immunocytochemistry and Immunohistochemistry

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
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Updated: Apr 5, 2026

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

Published on: March 30, 2020

Recent developments in multiplexing techniques for immunohistochemistry.

Angela R Dixon¹, Cédric Bathany, Michael Tsuei

¹a 1 Biomedical Engineering Department, College of Engineering, University of Michigan, Ann Arbor, MI 48109, USA.

Expert Review of Molecular Diagnostics

|August 21, 2015

Summary

This summary is machine-generated.

Recent advances in multiplex immunohistochemistry (IHC) enable high-resolution detection of low-abundance molecules. These techniques enhance rare tissue analysis and early disease detection in clinical research.

Keywords:

colorimetric multiplexing immunohistochemistry multiplex signal enhancement spatially-patterned multiplexing

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Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Published on: April 8, 2016

Area of Science:

Biomedical research
Molecular pathology
Diagnostic imaging

Background:

Immunohistochemistry (IHC) is crucial for analyzing tissue biomarkers.
High-resolution detection of low-abundance molecules is an ongoing challenge.
Multiplex IHC offers enhanced diagnostic capabilities.

Purpose of the Study:

To review recent multiplex immunohistochemistry (IHC) methods.
To assess their potential for clinical research and application.
To discuss signal enhancement technologies for IHC assays.

Main Methods:

Categorization of multiplex IHC into whole-section and spatially-patterned staining.
Discussion of signal enhancement technologies.
Evaluation of techniques for detecting 3-30 discrete antigens.

Main Results:

Multiplex IHC methods allow simultaneous detection of multiple antigens.
Techniques vary in resolution, sensitivity, and throughput.
Signal enhancement improves assay performance.

Conclusions:

Multiplex IHC is revolutionizing molecular pathology and diagnostics.
These methods offer significant advantages for rare tissue analysis and early disease detection.
Careful consideration of technique-specific advantages and tradeoffs is essential for clinical translation.