[Molecular analysis in 13 cases of chronic Philadelphia-positive myeloid leukemia]

Thirteen cases of Ph-positive CML have been studied from a cytogenetic and molecular point of view. All cases had Ph chromosome identified as t(9;22). Three of these cases were in blast crisis, and additional alterations to the Ph chromosome were found. The molecular study was made using the 3'bcr probe to analyze the ability to detect the translocation at the molecular level in bone marrow, peripheral blood, other tissues and in cases with mosaicism. Likewise we have evaluated the possibility to obtain molecular results from Carnoy-fixed samples which have been previously used in cytogenetic studies. In all cases we have detected rearrangement in the bcr region; this shows the interest and usefulness of this new methodology in the study of CML in different situations.