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Updated: Apr 5, 2026

Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins
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Protein-specific imaging of posttranslational modifications.

Wei Lin1, Ling Gao1, Xing Chen1

  • 1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.

Current Opinion in Chemical Biology
|August 21, 2015
PubMed
Summary

Imaging specific protein posttranslational modifications (PTMs) is challenging. Proximity-enabled strategies offer a solution by using dual labeling for nanometer proximity-dependent fluorescent signals, enabling visualization of PTMs in cells and tissues.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Imaging

Background:

  • Protein posttranslational modifications (PTMs) regulate diverse cellular functions.
  • Identifying specific PTMs on individual proteins is difficult due to their widespread occurrence.
  • Existing methods struggle with the specificity required for single-protein PTM analysis.

Purpose of the Study:

  • To review advancements in proximity-enabled strategies for protein-specific PTM imaging.
  • To highlight the methodological developments in this imaging approach.
  • To showcase applications in studying key PTMs like phosphorylation, glycosylation, and lipidation.

Main Methods:

  • Exploiting spatial proximity between a PTM and its modified protein.
  • Dual labeling of the protein and the PTM with distinct tags.
  • Generating nanometer proximity-dependent fluorescent signals for visualization.

Main Results:

  • Proximity-enabled strategies provide a powerful tool for PTM imaging.
  • This approach allows for visualization in single cells and tissue sections.
  • Recent advances have expanded the scope and applicability of these methods.

Conclusions:

  • Proximity-enabled imaging overcomes the challenges of PTM detection.
  • This technique facilitates the study of phosphorylation, glycosylation, and lipidation.
  • It offers a valuable approach for understanding protein function and regulation.