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The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
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Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
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An Nfic-hedgehog signaling cascade regulates tooth root development.

Yang Liu1, Jifan Feng2, Jingyuan Li3

  • 1Center for Craniofacial Molecular Biology, Ostrow School of Dentistry, University of Southern California, Los Angeles, CA 90033, USA Department of Prosthodontics, Peking University School and Hospital of Stomatology, Haidian District, Beijing 100081, People's Republic of China.

Development (Cambridge, England)
|August 22, 2015
PubMed
Summary
This summary is machine-generated.

A newly discovered Nfic-Hhip-Hh signaling pathway is essential for tooth root development. This pathway regulates apical papilla growth and ensures proper formation of dental roots.

Keywords:
MouseNficProliferationRoot developmentShh

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Tooth root development requires precise coordination between Hertwig's epithelial root sheath (HERS) and the apical papilla (AP).
  • The hedgehog (Hh) signaling pathway and Nfic are known regulators of tooth development, but their interplay in root formation is unclear.

Purpose of the Study:

  • To elucidate the relationship between Hh signaling and Nfic in mouse molar root development.
  • To identify the molecular mechanisms underlying apical papilla growth and tooth root formation.

Main Methods:

  • Histological staining of mouse molar sections to establish a developmental timecourse.
  • Utilizing Gli1 reporter mice to track Hh signaling activity.
  • Employing Hh inhibitors and transgenic mice to manipulate Hh signaling.
  • RNA sequencing and in situ hybridization to analyze gene expression.
  • Chromatin immunoprecipitation (ChIP) and RNAscope analyses to investigate molecular interactions.

Main Results:

  • Hh signaling is active in the AP and HERS during root development.
  • Aberrant Hh signaling (inhibition or constitutive activation) impairs AP cell proliferation and results in shorter roots.
  • Nfic(-/-) mice exhibit elevated Hh activity and downregulated Hhip, an Hh attenuator.
  • Nfic directly binds to the Hhip promoter region.
  • Hh inhibitor treatment partially rescues root development defects in Nfic(-/-) mice.

Conclusions:

  • An Nfic-Hhip-Hh signaling pathway is critical for regulating apical papilla growth and proper tooth root formation.
  • This pathway represents a key molecular mechanism governing dental root development.