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Updated: Apr 5, 2026

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
Development of methodology for identification the nature of the polyphenolic extracts by FTIR associated with
Fábio dos Santos Grasel1, Marco Flôres Ferrão2, Carlos Rodolfo Wolf3
1Departamento de Controle da Qualidade, Tanac S/A, Montenegro, RS, Brazil; Programa de Pós-graduação em Engenharia e Tecnologia de Materiais, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Abstract:
Tannins are polyphenolic compounds of complex structures formed by secondary metabolism in several plants. These polyphenolic compounds have different applications, such as drugs, anti-corrosion agents, flocculants, and tanning agents. This study analyses six different type of polyphenolic extracts by Fourier transform infrared spectroscopy (FTIR) combined with multivariate analysis. Through both principal component analysis (PCA) and hierarchical cluster analysis (HCA), we observed well-defined separation between condensed (quebracho and black wattle) and hydrolysable (valonea, chestnut, myrobalan, and tara) tannins. For hydrolysable tannins, it was also possible to observe the formation of two different subgroups between samples of chestnut and valonea and between samples of tara and myrobalan. Among all samples analysed, the chestnut and valonea showed the greatest similarity, indicating that these extracts contain equivalent chemical compositions and structure and, therefore, similar properties.
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