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Published on: October 30, 2016
Identification of clinically relevant Corynebacterium strains by Api Coryne, MALDI-TOF-mass spectrometry and
S Alibi1, A Ferjani2, O Gaillot2
1UR12/SP34 laboratoire de microbiologie et immunologie, CHU Farhat-Hached, Sousse, Tunisia; Faculté des sciences de Bizerte, université Carthage, Jarzouna Tunisia.
Abstract:
We evaluated the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) for the identification of 97 Corynebacterium clinical in comparison to identification strains by Api Coryne and MALDI-TOF-MS using 16S rRNA gene and hypervariable region of rpoB genes sequencing as a reference method. C. striatum was the predominant species isolated followed by C. amycolatum. There was an agreement between Api Coryne strips and MALDI-TOF-MS identification in 88.65% of cases. MALDI-TOF-MS was unable to differentiate C. aurimucosum from C. minutissimum and C. minutissimum from C. singulare but reliably identify 92 of 97 (94.84%) strains. Two strains remained incompletely identified to the species level by MALDI-TOF-MS and molecular approaches. They belonged to Cellulomonas and Pseudoclavibacter genus. In conclusion, MALDI-TOF-MS is a rapid and reliable method for the identification of Corynebacterium species. However, some limits have been noted and have to be resolved by the application of molecular methods.
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