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Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
10:51

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Published on: November 15, 2013

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Classical nuclear hormone receptor activity as a mediator of complex biological responses: a look at health and

Paul Michael Yen1

  • 1Cardiovascular and Metabolic Disorders Program, Duke-NUS Graduate Medical School, Singapore.

Best Practice & Research. Clinical Endocrinology & Metabolism
|August 26, 2015
PubMed

Insights

Nuclear hormone receptors (NHRs) are crucial transcription factors regulating genes involved in development and metabolism. Targeting NRs offers therapeutic potential for cancer and metabolic diseases.

Area of Science:

  • Endocrinology and Molecular Biology
  • Gene Regulation and Metabolism

Background:

  • Nuclear hormone receptors (NHRs) are a vast family of ligand-inducible transcription factors.
  • They regulate critical physiological processes including development, reproduction, and metabolism.
  • NHRs bind diverse ligands such as steroid hormones, thyroid hormone, vitamin D, and various metabolites.

Purpose of the Study:

  • To provide a comprehensive overview of nuclear hormone receptors.
  • To highlight their roles in gene regulation and physiological functions.
  • To discuss the therapeutic implications of targeting NRs.

Main Methods:

  • Literature review of NHRs, their ligands, and functions.
  • Analysis of NHRs' role in disease and drug development.
  • Exploration of genetic mechanisms underlying hormone resistance syndromes.

Main Results:

  • NHRs control gene expression in response to hormones and metabolites.
  • Orphan receptors, despite unknown ligands, are key metabolic regulators.
  • Targeting NRs is a promising strategy for treating cancer and metabolic disorders.

Conclusions:

  • NHRs are essential regulators of cellular and organismal homeostasis.
  • Understanding NHRs and their dysfunctions is vital for developing novel therapeutics.
  • NHR research continues to expand our knowledge of endocrine signaling and disease mechanisms.
Keywords:
antagonisthormone resistancemetabolismnuclear hormone receptortranscription

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