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Author Spotlight: Optimizing CFU Determination for Efficient Assessment of TB Vaccine Efficacy and Antigen Presentation Analysis
Published on: July 28, 2023
Diagnostic value of antibody responses to multiple antigens from Mycobacterium tuberculosis in active and latent
Muhammad Andrian Senoputra1, Beata Shiratori2, Fakhrial Mirwan Hasibuan1
1Division of Emerging Infectious Diseases, Graduate School of Medicine, Tohoku University, 21 Seiryo-machi, Aoba-ku, Sendai, 980-8574 Miyagi, Japan; Public Health Science Program, Faculty of Medicine, Universitas Padjadjaran, Jl. Eicjkman 38, Bandung, 40161, West Java, Indonesia.
Abstract:
We investigated the antibody responses to 10 prospective Mycobacterium tuberculosis (MTB) antigens and evaluated their ability to discriminate between latent (LTBI) and active pulmonary tuberculosis (TB). Our results indicate that plasma levels of anti-α-crystallin (ACR), antilipoarabinomannan, anti-trehalose 6,6'-dimycolate, and anti-tubercular-glycolipid antigen antibodies were higher in patients with active TB, compared to those in the LTBI and control subjects. No differences in the antibodies were observed between the control and LTBI subjects. Antibodies against the glycolipid antigens could not distinguish between Mycobacterium avium complex (MAC)-negative TB patients and MAC-infected LTBI individuals. The most useful serological marker was antibodies to ACR, with MAC-negative TB patients having higher titers than those observed in MAC-positive LTBI and control subjects. Our data indicate that antibody to ACR is a promising target for the serological diagnosis of patients with active TB patients. When dealing with antiglycolipid antibodies, MAC coinfection should always be considered in serological studies.
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