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Related Experiment Video

Updated: Apr 5, 2026

Oligopeptide Competition Assay for Phosphorylation Site Determination

Oligopeptide Competition Assay for Phosphorylation Site Determination

Published on: May 18, 2017

One assay for all: exploring small molecule phosphorylation using amylose-polyiodide complexes.

Xu C Duan¹, Huan Chen¹, Fang F Liu¹

¹Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University , Nanjing, Jiangsu 210095 China.

Analytical Chemistry

|August 27, 2015

Summary

This summary is machine-generated.

We developed a new assay to screen small molecule kinases. This method uses an amylose-polyiodide complex to detect kinase activity and determine substrate specificity, enabling high-throughput screening.

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Last Updated: Apr 5, 2026

Oligopeptide Competition Assay for Phosphorylation Site Determination

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Published on: May 18, 2017

Rapid High Throughput Amylose Determination in Freeze Dried Potato Tuber Samples

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Published on: October 14, 2013

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

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Published on: June 6, 2022

Area of Science:

Biochemistry
Enzymology
Assay Development

Background:

Kinase activity screening is crucial for drug discovery.
Existing methods for kinase substrate specificity profiling can be complex or limited.

Purpose of the Study:

To present a generic, high-throughput method for screening small molecule kinases.
To determine the substrate specificity of nucleotide- and sugar-kinases.

Main Methods:

Utilized sucrose synthase and glycogen synthase enzymes.
Leveraged the byproduct adenosine diphosphate (ADP) to form an amylose-polyiodide complex.
Quantified kinase activity via photometric detection of the blue complex.

Main Results:

Demonstrated the method's ability to profile known and novel kinases.
Successfully screened for substrate specificity using the developed assay.
Established a facile and widely available methodology.

Conclusions:

The amylose-polyiodide kinase assay is a versatile tool for substrate screening.
This method facilitates high-throughput analysis of small molecule kinase activity.
The assay has potential for profiling diverse kinase families.