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Related Experiment Video

Updated: Apr 5, 2026

Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice

Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice

Published on: July 23, 2017

Diabetes Is Reversed in a Murine Model by Marginal Mass Syngeneic Islet Transplantation Using a Subcutaneous Cell

Andrew R Pepper¹, Rena Pawlick, Boris Gala-Lopez

¹1 Clinical Islet Transplant Program, Alberta Diabetes Institute, University of Alberta, Edmonton, AB, Canada. 2 Sernova Corp. London, ON, Canada. 3 Department of Surgery, Robarts Research Institute, University of Western Ontario, ON, Canada. 4 Department of Surgery, University of Alberta, Edmonton, AB, Canada.

Transplantation

|August 27, 2015

Summary

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A novel implantable device, the Cell Pouch (CP), successfully enabled subcutaneous islet transplantation in mice, restoring glycemic control and offering a promising alternative site for diabetes therapy.

Area of Science:

Regenerative Medicine
Transplantation Biology
Diabetes Research

Background:

Islet transplantation is a key therapy for type 1 diabetes, but long-term function is limited.
Current intraportal transplantation has risks and limitations, necessitating alternative sites.
Subcutaneous islet transplantation has historically failed without supportive technology.

Purpose of the Study:

To evaluate the efficacy of a prevascularized implantable device (Cell Pouch) for subcutaneous islet transplantation.
To determine if the Cell Pouch can overcome previous failures of subcutaneous islet engraftment.
To assess the potential of the Cell Pouch as an alternative transplantation site for diabetes treatment.

Main Methods:

Syngeneic mouse islets were transplanted into pre-implanted Cell Pouches (CPs) in mice.

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Published on: April 13, 2011

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Last Updated: Apr 5, 2026

Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice

Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice

Published on: July 23, 2017

Intraportal Transplantation of Pancreatic Islets in Mouse Model

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Published on: May 5, 2018

A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation

A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation

Published on: April 13, 2011

Recipients were monitored for glycemic control and glucose tolerance post-transplantation.

Islet grafts within the CP were analyzed for insulin, glucagon, and vascularization.

Main Results:

Islets transplanted within the CP restored glycemic control in mice, comparable to traditional methods.
Subcutaneous islets transplanted without the CP failed to engraft.
The CP facilitated islet survival, vascularization, and hormone production.

Conclusions:

The Cell Pouch (CP) is biocompatible and creates a suitable environment for islet engraftment.
The CP offers a viable alternative site for islet transplantation, overcoming limitations of intraportal delivery.
This technology holds potential for future stem cell-based therapies for diabetes.