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Related Experiment Video

Updated: Jun 5, 2026

Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice
11:57

Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice

Published on: July 23, 2017

Nanofibrous Bioabsorbable Functionalized Scaffolds Augment Subcutaneous Islet Engraftment and Function in Mice.

Purushothaman Kuppan1,2, Chelsea Castro1,2, Joy Paramor1,2

  • 1Alberta Diabetes Institute, University of Alberta, Edmonton, Alberta, Canada.

Diabetes
|June 4, 2026
PubMed
Summary

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Bioabsorbable functionalized scaffolds (BAFS) create a vascularized subcutaneous niche for islet transplantation. These BAFS support long-term islet graft survival and improve diabetes management in mice.

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Endocrinology

Background:

  • The subcutaneous space is a potential site for islet transplantation to treat type 1 diabetes.
  • Developing a vascularized niche is crucial for successful islet engraftment and function.

Purpose of the Study:

  • To evaluate nanofibrous bioabsorbable functionalized scaffolds (BAFS) for creating a vascularized subcutaneous niche to support islet transplantation.
  • To assess the efficacy of BAFS in promoting vascularization and islet graft survival in a mouse model.

Main Methods:

  • Electrospun poly(lactic-co-glycolic acid) and gelatin scaffolds functionalized with vascular endothelial growth factor and laminin were implanted in the subcutaneous space of mice.
  • Vascularization was assessed by quantifying lectin, smooth muscle actin, and CD31 positive cells.

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Related Experiment Videos

Last Updated: Jun 5, 2026

Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice
11:57

Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice

Published on: July 23, 2017

Fat-Covered Islet Transplantation Using Epididymal White Adipose Tissue
06:39

Fat-Covered Islet Transplantation Using Epididymal White Adipose Tissue

Published on: May 25, 2021

Surface Engineering of Pancreatic Islets with a Heparinized StarPEG Nanocoating
05:35

Surface Engineering of Pancreatic Islets with a Heparinized StarPEG Nanocoating

Published on: June 23, 2018

  • Neonatal porcine islets were transplanted into diabetic mice with BAFS, and glycemic control was monitored.

    • Main Results:

    • BAFS significantly enhanced vascularization in the subcutaneous space compared to control groups.
    • Islet recipients with BAFS achieved normoglycemia faster and maintained higher serum porcine insulin levels.
    • The BAFS supported long-term islet graft survival, indicating successful engraftment and function.

    Conclusions:

    • Nanofibrous BAFS effectively create a vascularized subcutaneous niche that supports islet engraftment and long-term function.
    • This approach offers a promising and tunable alternative site for beta-cell replacement therapies in treating type 1 diabetes.