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Published on: September 15, 2023
MiR-30b suppresses tumor migration and invasion by targeting EIF5A2 in gastric cancer
Shu-Bo Tian1, Jian-Chun Yu1, Yu-Qin Liu1
1Shu-Bo Tian, Jian-Chun Yu, Wei-Ming Kang, Zhi-Qiang Ma, Xin Ye, Chao Yan, Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
Aim:
To elucidate the potential biological role of miR-30b in gastric cancer and investigate the underlying molecular mechanisms of miR-30b to inhibit metastasis of gastric cancer cells.
Methods:
The expression of miR-30b was detected in gastric cancer cell lines and samples by reverse transcription-polymerase chain reaction. CCK-8 assays were conducted to explore the impact of miR-30b overexpression on the proliferation of gastric cancer cells. Flow cytometry was used to examine the effect of miR-30b on the apoptosis. Transwell test was used for the migration and invasion assays. Luciferase reporter assays and Western blot were employed to validate regulation of putative target of miR-30b.
Results:
The results showed that miR-30b was downregulated in gastric cancer tissues and cancer cell lines and functioned as a tumor suppressor. Overexpression of miR-30b promoted cell apoptosis, and suppressed proliferation, migration and invasion of the gastric cancer cell lines AGS and MGC803. Bioinformatic analysis identified the 3'-untranslated region of eukaryotic translation initiation factor 5A2 (EIF5A2) as a putative binding site of miR-30b. Luciferase reporter assays and Western blot analysis confirmed the EIF5A2 gene as a target of miR-30b. Moreover, expression levels of the EIF5A2 targets E-cadherin and Vimentin were altered following transfection of miR-30b mimics.
Conclusion:
Our findings describe a link between miR-30b and EIF5A2, which plays an important role in mediating epithelial-mesenchymal transition.
Insights
MicroRNA-30b (miR-30b) is downregulated in gastric cancer and acts as a tumor suppressor. Its overexpression inhibits cancer cell proliferation and metastasis by targeting EIF5A2, a key factor in epithelial-mesenchymal transition.
Area of Science:
- Oncology
- Molecular Biology
- Gene Regulation
Background:
- Gastric cancer is a significant global health concern with high mortality rates.
- Metastasis is a primary driver of gastric cancer-related deaths.
- MicroRNAs (miRNAs) are emerging as critical regulators in cancer development and progression.
Purpose of the Study:
- To investigate the biological role of miR-30b in gastric cancer.
- To elucidate the molecular mechanisms by which miR-30b inhibits gastric cancer metastasis.
- To identify direct targets of miR-30b involved in gastric cancer progression.
Main Methods:
- Quantitative reverse transcription-polymerase chain reaction (RT-PCR) for miR-30b expression analysis.
- Cell proliferation assays (CCK-8), apoptosis assays (flow cytometry), and migration/invasion assays (Transwell).
- Luciferase reporter assays and Western blot to validate miR-30b targets and downstream effects.
Main Results:
- miR-30b expression was significantly downregulated in gastric cancer tissues and cell lines.
- Overexpression of miR-30b suppressed proliferation, induced apoptosis, and inhibited migration and invasion in gastric cancer cells.
- Eukaryotic translation initiation factor 5A2 (EIF5A2) was identified and validated as a direct target of miR-30b, influencing E-cadherin and Vimentin expression.
Conclusions:
- miR-30b functions as a tumor suppressor in gastric cancer.
- The miR-30b/EIF5A2 axis plays a crucial role in regulating epithelial-mesenchymal transition (EMT) in gastric cancer.
- miR-30b represents a potential therapeutic target for inhibiting gastric cancer metastasis.
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