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Updated: Apr 5, 2026

Optical Trapping of Nanoparticles

Optical Trapping of Nanoparticles

Published on: January 15, 2013

Macro-optical trapping for sample confinement in light sheet microscopy.

Zhengyi Yang¹, Peeter Piksarv², David E K Ferrier³

¹SUPA, School of Physics and Astronomy, University of St. Andrews, North Haugh, St. Andrews, KY16 9SS, UK.

Biomedical Optics Express

|August 27, 2015

Summary

This summary is machine-generated.

This study introduces a novel light sheet microscope using optical forces to trap samples, eliminating the need for immobilizing agents. This innovation enables imaging of live, mobile specimens with reduced photo-damage and expanded applications.

Keywords:

(110.0110) Imaging systems (110.0180) Microscopy (180.6900) Three-dimensional microscopy (350.4855) Optical tweezers or optical manipulation

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Optical Trapping of Nanoparticles

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Published on: January 15, 2013

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Area of Science:

Biophysics
Optical Engineering
Microscopy

Background:

Light sheet microscopy offers 3D imaging with low phototoxicity but typically requires sample embedding (e.g., agarose) or anesthesia.
Current methods can restrict live sample development and hinder imaging of mobile organisms.

Purpose of the Study:

To develop a light sheet microscopy technique that immobilizes samples using only optical forces.
To overcome limitations associated with traditional sample mounting and anesthesia in light sheet imaging.

Main Methods:

An integrated light sheet microscope was designed utilizing counter-propagating laser beams to generate optical forces.
Optical trapping was employed to hold specimens without physical contact or chemical agents.

Main Results:

Successfully trapped and acquired sectional images of tobacco plant cells.
Demonstrated optical trapping of living Spirobranchus lamarcki larvae for imaging.

Conclusions:

The optical trapping approach in light sheet microscopy eliminates the need for physical embedding or anesthesia.
This method significantly broadens the scope of live and mobile specimens amenable to high-resolution 3D imaging.