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Development of nuclear and chloroplast microsatellite markers for the endangered conifer Callitris sulcata
Shota Sakaguchi1, Guillaume Lannuzel2, Bruno Fogliani2
1Laboratory of Plant Evolution and Biodiversity, Department of General Systems Studies, Graduate School of Arts and Sciences, University of Tokyo, Komaba 1538902, Japan.
Premise Of The Study:
Microsatellite markers were developed for Callitris sulcata (Cupressaceae), an endangered conifer species in New Caledonia.
Methods And Results:
Using sequencing by synthesis (SBS) of an RNA-Seq library, 15 polymorphic nuclear and chloroplast microsatellite markers were developed. When evaluated with 48 individuals, these markers showed genetic variations ranging from two to 15 alleles and expected heterozygosity ranging from 0 to 0.881.
Conclusions:
These markers will be useful for examining the genetic diversity and structure of remaining wild populations and improving the genetic status of ex situ populations.
