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Related Experiment Videos

Obtaining Lissamine Green 1% Solution for Clinical Use.

Michael Stock1, David Salvay, Bradley Shoss

  • 1Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, MO.

Cornea
|August 28, 2015
PubMed
Summary

Ophthalmologists can create a 1% lissamine green solution using dye-impregnated strips and microcentrifuge tubes, ensuring compliance with pharmacy laws and reducing costs for vital staining.

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Area of Science:

  • Ophthalmology
  • Ophthalmic Pharmacy
  • Spectrophotometry

Background:

  • New compounding pharmacy laws necessitate cost-effective methods for using lissamine green.
  • Dye-impregnated strips represent the most economically viable approach for lissamine green application.

Purpose of the Study:

  • To determine the achievable concentration of lissamine green from a single commercially available dye-impregnated strip.
  • To ascertain if a 1% lissamine green concentration, optimal for vital staining, can be obtained using standard ophthalmology clinic supplies.

Main Methods:

  • A standard curve was created using a 1% compounded lissamine green solution.
  • Serial dilutions were prepared with three diluents and measured via spectrophotometry at 633 μm.
  • Various combinations of strips, solvent volumes, and absorption times were tested to achieve a 1% solution, alongside cost analyses.

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Main Results:

  • A single lissamine strip yielded a 0.17% ± 0.05% solution, significantly lower than the optimal 1% concentration.
  • A 1% lissamine green concentration was achieved using either 4 strips in 200 μL for 1 minute or 2 strips in 200 μL for 5 minutes.
  • The cost analysis favored the 2-strip method ($0.67) over the 4-strip method ($1.27).

Conclusions:

  • Single lissamine strips produce suboptimal concentrations for vital staining.
  • Ophthalmologists can prepare a 1% lissamine green solution using disposable microcentrifuge tubes, meeting optimal concentrations.
  • This method is both economical and compliant with state and national compounding regulations.