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Updated: Apr 4, 2026
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
[Sequence Analysis of A Novel HLA-B*13:68 Allele]
Bin Han1, Li Han1, Zhi-Hui Feng1
1Institute of Transfusion Medicine, Qingdao Blood Center, Qingdao 266071, Shandong Province, China.
Objective:
The purpose of this research was to identify a novel HLA-B allele in Chinese population.
Methods:
The HLA typing of bone marrow donors was performed by PCR-SBT. The ambiguous novel HLA allele was confirmed with GSSP method.
Results:
The sequence of a sample was different from all alleles in the HLA-B databases. The sequence analysis showed that it differed from the closet matching allele HLA-B*13:01:01 at one nucleotide substitution, 137 T > C in Exon2, which resulted in an amino acid change from Phenylalanine (Phe) to Serine (Ser) at codon 22.
Conclusion:
A novel allele was identified and named as HLA-B*13:68 by the WHO Nomenclature Committee.
