Ratio manipulating spectrophotometry versus chemometry as stability indicating methods for cefquinome sulfate...

Area of Science:

• Analytical Chemistry
• Spectroscopy
• Chemometrics

Background:

• Cefquinome sulfate (CFQ) analysis requires methods that can resolve it from degradation products.
• Existing methods like RP-HPLC can be time-consuming and costly.

Purpose of the Study:

• To develop and validate selective, rapid, and cost-effective spectrophotometric methods for CFQ determination.
• To analyze CFQ in the presence of its hydrolytic, oxidative, and photo-degradation products.
• To compare advanced chemometric methods with traditional ones.

Main Methods:

• Ratio difference, derivative ratio, and mean centering spectrophotometric methods.
• Concentration Residuals Augmented Classical Least Squares (CRACLS) and Partial Least Squares (PLS) regression.
• Validation using experimentally designed synthetic mixtures.

Main Results:

• Proposed spectrophotometric methods achieved selective CFQ determination (5.0-40.0 μg mL⁻¹).
• CRACLS and PLS successfully performed quantitative and qualitative analyses of CFQ and its degradation products.
• Methods were successfully applied to pharmaceutical formulations, showing cost-effectiveness compared to RP-HPLC.

Conclusions:

• Developed spectrophotometric and chemometric methods are suitable for selective CFQ analysis.
• These methods offer a simpler, faster, and more economical alternative to RP-HPLC.
• The study demonstrates the utility of advanced chemometrics in pharmaceutical analysis.