Related Experiment Video

Updated: Apr 4, 2026

Author Spotlight: Generation of Patient-Derived Podocytes from Skin Biopsies
08:52

Author Spotlight: Generation of Patient-Derived Podocytes from Skin Biopsies

Published on: May 26, 2023

3.3K

B7-1 Is Not Induced in Podocytes of Human and Experimental Diabetic Nephropathy

Elena Gagliardini1, Rubina Novelli1, Daniela Corna1

  • 1IRCCS - Istituto di Ricerche Farmacologiche Mario Negri, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, Bergamo, Italy;

Journal of the American Society of Nephrology : JASN
|August 31, 2015
PubMed

Insights

B7-1 is not induced in the kidneys of patients with diabetic nephropathy (DN). This finding does not support using abatacept to target B7-1 for treating DN, despite previous suggestions for this approach.

Area of Science:

  • Nephrology
  • Immunology
  • Diabetology

Background:

  • Diabetic nephropathy (DN) incidence is rising globally, with current therapies offering incomplete kidney protection.
  • Novel therapeutic strategies are crucial for managing progressive kidney disease in diabetes.
  • Previous research suggested B7-1 induction in podocytes could make abatacept a potential DN therapy.

Purpose of the Study:

  • To investigate B7-1 expression in human and experimental diabetic nephropathy (DN).
  • To evaluate the potential of targeting podocyte B7-1 for DN treatment.
  • To determine if abatacept is a viable therapeutic strategy for DN.

Main Methods:

  • Immunohistochemical analysis of kidney specimens from patients with DN and BTBR ob/ob mice (a type 2 diabetes model).
  • Utilized various antibodies to detect B7-1 expression.
  • Evaluated B7-1 expression independent of disease stage in human DN.

Main Results:

  • B7-1 was not found to be induced in the podocytes of patients with DN, regardless of disease stage.
  • B7-1 expression was also not observed in BTBR ob/ob mice, a model for type 2 diabetes.
  • These findings contradict previous assumptions about B7-1 in DN.

Conclusions:

  • The study does not support the use of abatacept for targeting podocyte B7-1 in the prevention or treatment of diabetic nephropathy (DN).
  • Further research into alternative therapeutic strategies for DN is warranted.
  • The efficacy of targeting B7-1 in DN requires re-evaluation based on these findings.
Keywords:
B7–1diabetic nephropathypodocyte

More Related Videos

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

825
Assessment of Kidney Function in Mouse Models of Glomerular Disease09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

18.9K

Related Concept Videos

Pathophysiology of Diabetes01:20

Pathophysiology of Diabetes

Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia. The four categories of diabetes are type 1 diabetes, type 2 diabetes, other specific types of diabetes, and gestational diabetes.
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility,...
4.4K
Renal Corpuscle01:20

Renal Corpuscle

The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous...
9.1K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
17.0K
Chronic Kidney Disease II: Clinical Manifestations01:24

Chronic Kidney Disease II: Clinical Manifestations

Chronic Kidney Disease (CKD) progressively impairs multiple body systems due to the accumulation of uremic toxins, which disrupt cellular functions across various organs.Neurologic symptomsNeurologic symptoms often arise early in CKD, as uremic toxin buildup drives changes in cognitive and motor functions. Patients frequently experience fatigue, headache, confusion, difficulty concentrating, and, in severe cases, seizures. Peripheral neuropathy commonly manifests as burning sensations in the...
970
Nephrons01:10

Nephrons

The kidneys are intricate organs with millions of working units known as nephrons. Each nephron features two major structures: the renal corpuscle, which facilitates blood plasma filtration, and the renal tubule, which handles the glomerular filtrate. Blood supply is directly linked to the nephrons. The renal corpuscle consists of the glomerulus, a capillary network, and the Bowman's capsule, a double-walled epithelial structure that encases the glomerulus. The filtering of blood plasma...
9.1K