Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

54
Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
54

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comprehensive Insight into the Emerging World of Carbon Dots for Applications in Energy and Metal Ion Sensing.

Precision chemistry·2026
Same author

Primary Uterine Primitive Neuroectodermal Tumour Mistaken for Leiomyosarcoma in an Adolescent Girl: A Very Rare Case With Many Diagnostic and Therapeutic Challenges.

Cureus·2026
Same author

Moon Regolith Simulant-Based All-3D-Printed Triboelectric Nanogenerator for Effective Mechanical Energy Conversion.

Energy & fuels : an American Chemical Society journal·2026
Same author

Efficient adsorption of perfluorooctane sulfonate and perfluorobutane sulfonic acid from aqueous solution onto amine-functionalized diatomite granules.

Scientific reports·2026
Same author

Deciphering secondary metabolite potentials of halophilic marine-derived <i>Aspergillus ruber</i>.

3 Biotech·2026
Same author

Advancements in keratoplasty: Exploring newer techniques and imaging modalities for enhanced surgical outcomes.

Saudi journal of ophthalmology : official journal of the Saudi Ophthalmological Society·2025

Related Experiment Video

Updated: Apr 4, 2026

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
07:22

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

17.8K

Microbial Selenium Nanoparticles (SeNPs) and Their Application as a Sensitive Hydrogen Peroxide Biosensor.

Kumar Suranjit Prasad1, Jayraj V Vaghasiya2, Saurabh S Soni2

  • 1Department of Environmental Studies, Faculty of Science, The M. S. University of Baroda, Vadodara, 390002, Gujarat, India. suranjit@gmail.com.

Applied Biochemistry and Biotechnology
|August 31, 2015
PubMed
Summary
This summary is machine-generated.

Bacillus pumilus sp. BAB-3706 enzymes efficiently synthesized stable selenium nanoparticles (SeNPs). These microbial SeNPs were used to create a sensitive biosensor for detecting hydrogen peroxide (H2O2).

Keywords:
BiosensorEDAXHydrogen peroxideSelenium nanoparticlesTEMUV-vis spectroscopy

More Related Videos

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
03:55

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

Published on: June 27, 2022

4.4K
An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium
09:33

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

Published on: December 17, 2018

10.9K

Related Experiment Videos

Last Updated: Apr 4, 2026

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
07:22

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

17.8K
A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
03:55

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

Published on: June 27, 2022

4.4K
An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium
09:33

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

Published on: December 17, 2018

10.9K

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Electrochemistry

Background:

  • Environmental bacteria like Bacillus pumilus sp. BAB-3706 can produce enzymes.
  • Selenium nanoparticles (SeNPs) have unique properties for biosensing applications.

Purpose of the Study:

  • To synthesize stable selenium nanoparticles (SeNPs) using Bacillus pumilus sp. BAB-3706 enzymes.
  • To develop a sensitive hydrogen peroxide (H2O2) biosensor using these microbial SeNPs.

Main Methods:

  • Cell-free extract from Bacillus pumilus sp. BAB-3706 was used for SeNPs synthesis.
  • Characterization of SeNPs using UV-vis, TEM, EDAX, FT-IR, and XRD.
  • Modification of an indium tin oxide (ITO) electrode with SeNPs for H2O2 detection via cyclic voltammetry and amperometry.

Main Results:

  • Stable colloidal SeNPs were successfully synthesized and characterized.
  • The SeNP-modified electrode showed a sensitive amperometric response to H2O2.
  • The biosensor exhibited a linear detection range of 5 to 600 mM with a low limit of detection (3.00 μM).

Conclusions:

  • Microbial SeNPs are effective for fabricating biosensors.
  • The developed H2O2 biosensor is low-cost and highly sensitive.
  • This approach offers a promising method for H2O2 detection.