Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

7.4K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
7.4K
What is Genetic Engineering?00:49

What is Genetic Engineering?

81.5K
Overview
81.5K
Synthetic Biology02:55

Synthetic Biology

5.8K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
5.8K
Diversity in Cell Signaling Responses01:22

Diversity in Cell Signaling Responses

8.7K
The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity. 
Graded and Abrupt Responses
Some signaling systems generate...
8.7K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

7.9K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.9K
Combinatorial Gene Control02:33

Combinatorial Gene Control

9.9K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Resilience of recombinant antibiotic resistance gene-containing plasmids against common cell culture disposal methods.

Cell reports methods·2026
Same author

Genetic Markers Remain Detectable in Genetically Engineered Microbes Biocontained with a CRISPR Kill Switch.

Environmental science & technology·2026
Same author

Elucidating biodegradation of dimethyl terephthalate by two <i>Rhodococcus</i> strains for its valorization applications.

Metabolic engineering communications·2026
Same author

Elucidation of odd-chain dicarboxylate metabolism in Acinetobacter baylyi and application to polyethylene upcycling.

Trends in biotechnology·2026
Same author

Engineering microbial consortia for mixed plastic upcycling.

Nature communications·2025
Same author

Conditional guide RNA deactivation by mRNA and small molecule triggers in Saccharomyces cerevisiae.

New biotechnology·2025
Same journal

Upcycling food processing byproducts via advanced yeast fermentation.

Current opinion in biotechnology·2026
Same journal

Microbial C1 assimilation pathways for chemical synthesis: from native metabolism to synthetic design.

Current opinion in biotechnology·2026
Same journal

Medicinal plants fermentation: current knowledge and perspectives.

Current opinion in biotechnology·2026
Same journal

Fermented foods: lessons learned from metagenomics.

Current opinion in biotechnology·2026
Same journal

Microfluidic platforms for the transient transfection of mammalian cells: recent developments and challenges.

Current opinion in biotechnology·2026
Same journal

Harvesting insights from recent advances in yeast genomics for predictable and precision wine fermentation.

Current opinion in biotechnology·2026
See all related articles

Related Experiment Video

Updated: Apr 4, 2026

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
11:23

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

10.9K

Programmable genetic circuits for pathway engineering.

Allison Hoynes-O'Connor1, Tae Seok Moon1

  • 1Energy, Environmental and Chemical Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.

Current Opinion in Biotechnology
|September 1, 2015
PubMed
Summary
This summary is machine-generated.

Synthetic biology offers powerful tools for metabolic engineering, but requires developing diverse sensors and programmable circuits. Advances in CRISPR and modeling pave the way for future genetic control of metabolic pathways.

More Related Videos

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

2.9K
Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

2.4K

Related Experiment Videos

Last Updated: Apr 4, 2026

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
11:23

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

10.9K
Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

2.9K
Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

2.4K

Area of Science:

  • Synthetic biology
  • Metabolic engineering
  • Genetic control

Background:

  • Synthetic biology presents significant opportunities for advancing the genetic control of metabolic pathways.
  • Key challenges include the need for diverse, well-characterized sensors and robust programmable circuits.
  • Integrating multi-gene targeting with metabolic sensors and complex logic is crucial.

Purpose of the Study:

  • To outline the challenges and opportunities in applying synthetic biology to metabolic engineering.
  • To highlight the essential components for achieving precise genetic control of metabolic pathways.
  • To underscore the potential of synthetic biology in revolutionizing metabolic engineering.

Main Methods:

  • Development of diverse metabolite-sensing and condition-sensing promoters.
  • Construction of robust, programmable genetic circuits for input-output regulation.
  • Integration of multi-gene targeting strategies with metabolic sensors and logic circuits.
  • Utilization of tools such as CRISPR/Cas systems, in silico modeling, and dynamic sensor-regulators.

Main Results:

  • Progress has been made in creating essential components like novel sensors and regulatory circuits.
  • The integration of advanced tools like CRISPR/Cas and computational modeling is enabling more complex designs.
  • These advancements provide a solid foundation for future research and development.

Conclusions:

  • Synthetic biology holds immense promise for transformative advances in metabolic engineering.
  • Overcoming current challenges in sensor development and circuit design is key to realizing this potential.
  • Future research leveraging advanced tools will likely lead to significant breakthroughs in metabolic pathway control.