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Related Experiment Video

Updated: Apr 4, 2026

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer

Published on: September 22, 2015

Programmed synthesis of three-dimensional tissues.

Michael E Todhunter^1,2, Noel Y Jee^1,3, Alex J Hughes¹

¹Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California, USA.

|September 1, 2015

Summary

This summary is machine-generated.

This study introduces DNA-programmed assembly of cells (DPAC), a novel method for creating organoid-like tissues with precise control over size, shape, and cellular composition for in vitro research.

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Area of Science:

Biotechnology
Tissue Engineering
Cell Biology

Background:

Reconstituting tissues from cells is crucial for in vitro modeling of biological processes like morphogenesis, homeostasis, and disease.
Existing methods face limitations in precisely controlling tissue architecture and cellular organization.

Purpose of the Study:

To introduce DNA-programmed assembly of cells (DPAC), a novel method for engineering organoid-like tissues.
To enable precise control over tissue size, shape, cellular composition, and spatial heterogeneity.
To facilitate the study of collective cell behaviors under varied conditions.

Main Methods:

DPAC utilizes dissociated cells functionalized with DNA 'Velcro' for specific, reversible adhesion.
DNA-patterned substrates act as removable templates for layer-by-layer tissue assembly in 3D.
DNase is used to release assembled microtissues, which are then embedded in extracellular matrix (ECM) gels.

Main Results:

DPAC successfully reconstitutes multicellular organization with programmed parameters.
The method allows for single-cell spatial resolution in positioning cell subpopulations.
DPAC can generate centimeter-long tissue cultures and facilitates exploration of ECM composition and cell interactions.

Conclusions:

DPAC offers a powerful new platform for building complex, organoid-like tissues in vitro.
This technology enables detailed investigation into how microenvironmental factors influence cellular behavior and tissue development.
DPAC advances the field of tissue engineering and in vitro disease modeling.